anti-lamin c (Developmental Studies Hybridoma Bank)

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Developmental Studies Hybridoma Bank anti-lamin c
Anti Lamin C, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lamin+a+c/anti-Lamin+C/custom%40lc28%2E26%4042421656
Average 96 stars, based on 27 article reviews

anti-lamin c - by Bioz Stars, 2026-10

96/100 stars

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Western Blot:

Article Title: EWS-FLI1 and Activator Protein-1 (AP-1) Reciprocally Regulate Extracellular-Matrix Proteins in Ewing sarcoma Cells.
Article Snippet: Soluble nuclear, chromatin-bound, and cytoplasmic fractionation was performed using the Subcellular Protein Fractionation Kit for Cultured Cells (ThermoFisher Scientific) according to manufacturer’s instructions. .. Antibodies to the following proteins were used in the immunoblots: c-Jun (60A8, Cell Signaling Technology, #9165, 1:1000; RRID:AB_2130165), FLI1 (Abcam, #ab133485, 1:1000, RRID:AB_2722650); c-Fos (Santa Cruz #sc-271243, 1:100; RRID:AB_10610067), FOSL2/Fra2 (Cell Signaling Technology, #19967, 1:1000; RRID:AB_2722526), Lamin A/C (Developmental Studies Hybridoma Bank, #MANLAC1, 1:100; RRID:AB_2618203), actin (Cell Signaling Technology, #4970, 1:5000; RRID:AB_2223172), vinculin (Proteintech, 26520-1-AP, 1:5000; RRID:AB_2868558), alpha tubulin (Proteintech Cat #66031-1-Ig, 1:5000, RRID:AB_11042766), vimentin (Developmental Studies Hybridoma Bank, #AMF-17b, 1:1000; RRID:AB_528505), FN1 (Cell Signaling Technology, #26836 (also 26836BF), 1:1000, RRID:AB_2924220), Histone H3 (Cell Signaling Technology, #4499 (also 4499S, 4499P, 4499L), 1:1000, RRID:AB_10544537), and FAP (E1V9V, Cell Signaling Technology, #66562, 1:1000, RRID:AB_2904193). ..

Article Title: EWS-FLI1 and Activator Protein-1 (AP-1) Reciprocally Regulate Extracellular-Matrix Proteins in Ewing sarcoma Cells
Article Snippet: Soluble nuclear, chromatin-bound, and cytoplasmic fractionation was performed using the Subcellular Protein Fractionation Kit for Cultured Cells (ThermoFisher Scientific) according to manufacturer’s instructions. .. Antibodies to the following proteins were used in the immunoblots: c-Jun (60A8, Cell Signaling Technology, #9165, 1:1000; RRID:AB_2130165), FLI1 (Abcam, #ab133485, 1:1000, RRID:AB_2722650); c-Fos (Santa Cruz #sc-271243, 1:100; RRID:AB_10610067), FOSL2/Fra2 (Cell Signaling Technology, #19967, 1:1000; RRID:AB_2722526), Lamin A/C (Developmental Studies Hybridoma Bank, #MANLAC1, 1:100; RRID:AB_2618203), actin (Cell Signaling Technology, #4970, 1:5000; RRID:AB_2223172), vinculin (Proteintech, 26520-1-AP, 1:5000; RRID:AB_2868558), alpha tubulin (Proteintech Cat #66031-1-Ig, 1:5000, RRID:AB_11042766), vimentin (Developmental Studies Hybridoma Bank, #AMF-17b, 1:1000; RRID:AB_528505), FN1 (Cell Signaling Technology, #26836 (also 26836BF), 1:1000, RRID:AB_2924220), Histone H3 (Cell Signaling Technology, #4499 (also 4499S, 4499P, 4499L), 1:1000, RRID:AB_10544537), and FAP (E1V9V, Cell Signaling Technology, #66562, 1:1000, RRID:AB_2904193). .. Following SDS-PAGE separation, gel contents were transferred to polyvinylidene difluoride membranes (Millipore, Darmstadt, Germany).

Saline:

Article Title: High PER1 expression is associated with STK11 mutation and clinical biomarkers of immunotherapy resistance in lung adenocarcinoma.
Article Snippet: Membranes were blocked in TBS-T containing 5% non-fat milk powder (Cell Signaling, 9999; Apex, 20–241) and 5% BSA (Cell Signaling, 9998). .. The following primary antibodies were diluted in 5% BSA in tris-buffered saline (Cell Signaling, 12498S) supplemented with 0.01% Tween-20 (TBS-T; ChemCruz, sc-29113B) and used at the indicated dilutions: PER1 (1:1000, Boster Bio, A00876, RRID:AB_3086701), LKB1 (1:1000, Cell Signaling, #3050, RRID:AB_823559), JAG1 (1:1000, Cell Signaling, #70,109, RRID:AB_2799774), pAMPK-T172 (1:1000, Cell Signaling, #2535, RRID:AB_331250), AMPK (1:1000, Cell Signaling, #2532, RRID:AB_330331), Actin (1:500, DSHB, JLA20, RRID:AB_528068; 1:1000, Sigma-Aldrich, A5441, RRID:AB_476744), Lamin A/C (1:1000, DSHB, MANLAC3(4C10), RRID:AB_2618205). .. The following HRP-conjugated secondary antibodies were diluted in 5% non-fat milk in TBS-T at a 1:3000 dilution: anti-Rabbit (Boster Bio, BA1054, RRID:AB_2734136) and anti-Mouse (Fisher Scientific, 62–652-0, RRID:AB_2533947).

Article Title: High PER1 expression is associated with STK11 mutation and clinical biomarkers of immunotherapy resistance in lung adenocarcinoma
Article Snippet: Membranes were blocked in TBS-T containing 5% non-fat milk powder (Cell Signaling, 9999; Apex, 20–241) and 5% BSA (Cell Signaling, 9998). .. The following primary antibodies were diluted in 5% BSA in tris-buffered saline (Cell Signaling, 12498S) supplemented with 0.01% Tween-20 (TBS-T; ChemCruz, sc-29113B) and used at the indicated dilutions: PER1 (1:1000, Boster Bio, A00876, RRID:AB_3086701), LKB1 (1:1000, Cell Signaling, #3050, RRID:AB_823559), JAG1 (1:1000, Cell Signaling, #70,109, RRID:AB_2799774), pAMPK-T172 (1:1000, Cell Signaling, #2535, RRID:AB_331250), AMPK (1:1000, Cell Signaling, #2532, RRID:AB_330331), Actin (1:500, DSHB, JLA20, RRID:AB_528068; 1:1000, Sigma-Aldrich, A5441, RRID:AB_476744), Lamin A/C (1:1000, DSHB, MANLAC3(4C10), RRID:AB_2618205). .. The following HRP-conjugated secondary antibodies were diluted in 5% non-fat milk in TBS-T at a 1:3000 dilution: anti-Rabbit (Boster Bio, BA1054, RRID:AB_2734136) and anti-Mouse (Fisher Scientific, 62–652-0, RRID:AB_2533947).

Cell-Signaling:

Article Title: Inhibition of XPO1 by selinexor enhances terminal erythroid maturation through modulation of HSP70 trafficking in severe β 0 -thalassemia/HbE
Article Snippet: The membrane was blocked overnight using 5%w/v skimmed milk in 1XPBST (Himedia, Maharashtra, India) prior to immunoblotting. .. Target proteins were detected using human-specific primary antibodies against exportin-1/CRM1 (#46249; Cell Signaling, Danvers, MA), HSP70 (#4876, Cell Signaling), GATA-1 (#4591; Cell Signaling), lamin A/C (MANLAC1(4A7); DSHB, Iowa City, IA), and GAPDH (ABS16; Merck KGaA, Darmstadt, Germany), and HRP-conjugated species-specific secondary antibodies including goat anti-rabbit IgG (W401B; Promega, Madison, WI) and goat anti-mouse IgG (W402B; Promega). .. Chemiluminescent signals were developed using Immobilon Forte Western HRP substrate (Millipore, Burlington, MA) and detected by exposing to X-ray films.

Transcranial Magnetic Stimulation:

Incubation:

Article Title: Curbing Breast Cancer by Altering V-ATPase Action on F-Actin, Heterochromatin, ETV7 and mTORC2 Signaling.
Article Snippet: Cells were quenched with Phosphate Buffer Saline (PBS) and 50 mM NH4Cl (Sigma-Aldrich, 213330) for 10 minutes, permeabilized with 0.1% PBS- Triton for 10 minutes, and blocked with 1% PBS-BSA (Sigma-Aldrich, A2153) for 15 minutes. .. Samples were incubated with primary antibodies against V-H+-ATPase subunit c (Developmental Studies Hybridoma Bank or DSHB, 224-256-2-s, 1:50 dilution), ETV7 (DSHB PCRP-ETV7-1A1-s, 1:50), lamin A/C (DSHB MANLAC1(4A7), 1:50), and HP1α for heterochromatin (Cell Signaling Technology or CST, 2616, 1:100) for 1 hour at room temperature. .. The coverslips were then washed 7 times and incubated with secondary antibodies (antimouse R37121, or anti-rabbit R37117, ThermoFisher Scientific) conjugated with alexa fluor 594, and alexa fluor phalloidin 488 (dilution 1:100 in PBS-BSA, Molecular probes by Life Technologies, A12379) that specifically stains F-actin, for 1 hour.

other:

Article Title: Inhibition of XPO1 by selinexor enhances terminal erythroid maturation through modulation of HSP70 trafficking in severe β0-thalassemia/HbE.
Article Snippet: The membrane was blocked overnight using 5%w/v skimmed milk in 1XPBST (Himedia, Maharashtra, India) prior to immunoblotting.

Review